Technical Publications

We invite you to learn more about the research conducted by Vetanco on promoting animal health.

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    Nature has always produced almost every organic substance known. The plant kingdom is responsible for contributing with secondary metabolites administration; many of them offer a great added value due to their application in medicine, cosmetics, aliments and agrochemicals. Plants have their own defenses that normally protect them from other plants and predators.

    These defenses have a chemical nature and, generally, involve substances of secondary metabolism (CROTEAU et al., 2000; PINTO et al., 2002).

    Secondary products have an important role in plants’ adaptation to their environment. These molecules contribute to the interaction of the plants with different ecosystems (AERTS et al., 1991; HARBORNE, 1988). These products increase the survival probability, because they are responsible for different biological activities such as antibiotic, antifungal and antiviral in order to protect the plants from the pathogens, and some of them present antigerminative or toxic activities for other plants (LI et al., 1993). The concept of secondary metabolism has been briefly defined as less abundant compounds, with a frequency of less than 1% of the total carbon, due to their storage in organs or in specific cells. The primary metabolism is described as the vital functions for the survival of the plant, such as cell division, growth, breathing, storage and reproduction.

    The phytogenic additives are substances mainly volatile and lipophilic (SIMÕES C.M.O., SPITZER V., 1999), the majority of which are terpene hydrocarbons, simple alcohols, ketones, phenols and esters with pharmacologically active compounds. These additives present a significant variation in the chemical composition of the vegetal, depending on climate conditions, incidence of light, phase of harvest, localization or storage conditions (APPLEGATE et al., 2010).

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  • Enzymatic inactivation of mycotoxins

    In the mid-seventeenth century it was already known that stomach secretions were responsible for meat digestion and that saliva was, partially, in charge of conversion of starch into sugar. However, the mechanism responsible for those transformations was unknown. Two centuries later (XIX), the enzymatic mechanism was discovered and, it was not until the 1930s that the first enzymes were isolated. Since that moment, different functions have been discovered; functions that go far beyond nutrients digestion.

    In 1966, CIEGLERA et. al, tested approximately 1.000 microorganisms, among which were: yeasts, molds, fungal spores, actinomycetes, bacteria and algae, in order to evaluate their ability to destroy or transform the aflatoxin B1 and G1. Among all the agents studied, only one bacteria, Flavobacterium NRRL B-184, removed the aflatoxin from the solution. According to research studies on ducks (ducklings), the detoxification of aflatoxin solutions by B-184 was complete, without the production of new toxins.

    With the growing relevance of mycotoxins control in the animal production chain, this was a great opportunity: enzymatic inactivation or detoxification of mycotoxins. The detoxification of mycotoxins has been studied for a long time; the first reports of mycotoxins biotransformation in microorganisms were published in the 1960s. From a practical perspective, the first important result was discovered in the mid-1980s, when the inactivation capacity of T-2 toxin was proved. It was also proved that some organisms secrete enzymes that can cleave mycotoxins in specific regions, generating sub products with no toxicity or with low toxicity.

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  • Herbanoplex® CP: a unique pathosupressor phytocompound for preventing necrotic enteritis

    Antibiotic free poultry production can be realized with the application of alternative gut health stabilizers. For gut stabilization, different feed additives are applied during poultry rearing:

    – organic acids are the most commonly used additives for gut stabilization in the broilers diet, with well-defined antimicrobial activities and also a pH reducing ability,
    – trace elements and minerals can also be used, which possess antibacterial activities and can improve the immune status of the birds resulting in higher feed conversions,
    – probiotics can be added to the diet, which help in establishing a normal flora,
    – prebiotics can be used, which enhance the growth of the normal microbiome,
    – synbiotics can be applied, which are a combination of probiotics and prebiotics,
    – phytocompounds can be implemented into the broilers diet, which possess antimicrobial activities, meaning that in case of all phytocompounds, minimal inhibition concentrations (MICs) and killing dosages (CIDs) can be defined.

    All the above mentioned feed additive categories have important place in the broiler industry. In many cases they are used in combinations, taking advantage of the attributes of each group. For example, in the case of synbiotics, these compounds can increase the individually obtained beneficial effect of both probiotics and prebiotics. However, phytocompounds seem to be the sole feed additive category that may reduce the use of antibiotics if their inclusion in the broiler’s diet becomes more widespread. This also means that phytocompounds alone or in combination with
    other feed additives represent true alternatives to antibiotics.

    Antibiotic resistance is a growing problem in both animal husbandry and human healthcare. Therefore, governments – with subsidies and/or prohibitions – are calling for a reduction in antibiotic use. Most recent studies show a strong correlation between the antibiotic load in animal husbandry and the human antibiotic usage: namely reducing the antibiotic usage in animal husbandry also results in reduced human antibiotic applications.

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  • Evaluation of intestinal health in broiler chickens treated with Herbanoplex® CP and challenged with clostridium perfringens

    Enteric illnesses have a strong impact on the poultry industry, because its negative effects reduce production and increase both mortality and zoonotic risks.

    Among the most relevant pathologies, the necrotic enteritis (NE) stands out because it generates high costs for the poultry industry; annually, about 2 billion dollars are spent worldwide as a consequence of this illness that produce a growth delay and the death of birds.

    Necrotic enteritis is hard to be reproduced experimentally by using Clostridium perfringens isolated (LEE et al., 2011). To achieve this, we must combine elements that help to develop Clostridium perfringens in animals’ intestine, such as a high-protein diet, a high-rate of starch, immunosuppressive factors, factors that promote intestinal lesions and a sample of a viral strain of Clostridium perfringens for the challenges.

    Necrotic Enteritis has presented a higher number of cases since the prohibition of antimicrobial growth promoters (AGPs) by the European Union in 2006 (VAN IMMERSEEL et al., 2016). Since then, different control measures have been taken in order to replace AGPs.

    Vetanco, in partnership with the University of the State of Londrina (UEL), evaluated the product HERBANOPLEX® CP in 100 broiler chicken, male and one day-old, housed in experimental cages in the Laboratory of Infectology of the University of the State of Londrina UEL. The birds were immunosuppressed with a vaccine for Gumboro disease, challenged with oocysts of Eimeria and, in the sequence, challenged with Clostridium perfringens ,10⁸ UFC/ml.

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  • Detoxifying activity of Detoxa Plus®

    The following work is a summary and interpretation of the scientific article published in the journal TOXINS NUMBER 8, on October 15, 2019.

    Mycotoxins are toxic and complicated secondary active biological metabolites produced by filamentous fungal species, mainly Aspergillus, Fusarium and Penicillium [1]. When animals consume feeds contaminated with mycotoxins, they suffer from a series of toxic effects, such as decreased feed intake, reduced body weight gain, diarrhea, vomiting, as well as liver and kidney pulmonary edema [2]. Many different strategies, such as thermal inactivation, irradiation, physical dilution, and anti-mycotoxin products (adsorbents or elimination reagents), are used in feed mills and farms to reduce mycotoxin concentration of feedstuffs and feeds.

    The addition of anti-mycotoxin products in feed is currently an effective strategy for detoxifying animal feeds. There are two major types of anti-mycotoxin feed additives, i.e., adsorbents and elimination reagents. Mycotoxin adsorbents work by preventing the absorption of mycotoxins by the gastrointestinal tract of the animal by adsorbing the toxins to their surfaces. Adsorbents are either inorganic (e.g., bentonites and hydrated sodium calcium aluminosilicate) or organic products (yeast cell membrane) [4,5]. Enzyme reagents (EDRs) aim to alter the toxic chemical structure of the mycotoxins and further reduce their toxicity. They generally take the forms of the whole bacterium, yeast cultures or specifically extracted components, such as enzymes.


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  • Result of a treatment combining a phytobiotic, a prebiotic and organic acids with yeast wall for clostridiosis control in laying hens

    Herbanoplex CP; Gamaxine and Uniwall MOS

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  • Evaluation of long term immunity and protection against Salmonella spp by orally administrated inactivated vaccine

    Salmonella is the leading cause of foodborne infections and is a major public health concern worldwide. Poultry meat and eggs are a major reservoir of foodborne Salmonella serovars and vaccination against the pathogen is one of the important measures to curb infectious outbreaks and reduce antibiotic use, currently a major concern for consumers. In this study we evaluated the efficacy of a commercially available inactive, orally administered, subunit vaccine (Biotech Vac Salmonella) and determined if the immune response was protective and persistent (long-term immunity) against mobile Salmonella spp. in two separate commercial layer hen flocks (n=120,000 layers/lot) located in Pergamino, Buenos Aires, Argentina.

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  • Intestinal microflora stabilization with combining prebiotic effect and antimicrobial

    Phytobiotics, as it is demonstrated under both in vitro and in vivo conditions, can address gut health challenges in the poultry industry and can be used as novel growth promoters. Traditional herbs play an important role in eliminating pathogenic infections, represent a novel solution for antibiotic resistance at an affordable price and serve the consumer’s need for antibiotic free production. Combining herbal plant extracts with an optimal prebiotic allows products to work through multiple modes of action and ensures normal growth of the commensal microflora while inhibiting the growth of undesired pathogens and enhancing production parameters.

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  • Protection conferred by a subunit Salmonella vaccine against Salmonella Infantis in broiler chickens

    Salmonella infection is a major cause of bacterial enteric illness in both humans and animals.  This foodborne pathogen is often associated with poultry production and contaminated poultry products. Currently, vaccination is one of the main strategies to control Salmonella in commercial poultry farms avoiding the use of antimicrobials while improving food safety for consumers.  We have previously tested the efficacy of a commercially available subunit vaccine (Biotech Vac Salmonella) to protect against Salmonella enteritidis.

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  • Evaluation of a water applied biopromotor to improve production status and control Salmonella spp in commercial broiler chickens

    Currently, the world’s population is more conscious than ever about diseases associated with animal production especially foodborne pathogens that can directly affect public health.   There has long been a need to reduce Salmonella contamination in commercial poultry production while maintaining favorable production parameters. We have developed a water applied additive biopromotor composed of inactivated bacillus strains, selected for their ability to produce molecules which improve mucosal integrity and reduce gram negative bacteria, and yeast cell wall extracts as an alternative strategy for controlling foodborne pathogens and improving production parameters.

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  • Effects of a water applied biopromotor and feed applied MOS on the prevalence of Colibacillosis and egg production in commercial heavy breeders

    Commercial poultry production farms are under increasing pressure to reduce the use of antibiotics while maintaining health standards and pathogen control.  Currently, there is an increased need for the development of effective products that serve as alternatives to antibiotics. We have developed a water applied biopromotor through careful selection of different types of prebiotics strategies (inactivated fermented Bacillus subtilis fragments and yeast cell wall extracts) that improves mucosal integrity and controls gram negative pathogens, while increasing productivity parameters and health status of the birds.

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    Necrotic Enteritis (NE) is caused by Clostridium perfringens (CP).  It is characterized by the sudden appearance of signs, which are rapid weakness followed by death; Conuent areas of necrosis can be observed in the mucous membrane of the small intestine. The use of Growth Promoter Antibiotics (APC) has been the main strategy for the control of NE. The objective of this work was to evaluate the efficacy in the eld of a combined treatment with a phytobiotic (1), a prebiotic (2) and organic acids with yeast wall (3) to control CP in a laying hen farm with a characteristic repeated history of NE outbreaks.

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    The costs of animal diseases and societal benefits from healthy animals.

    IFAH annual report 2012

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  • Pathogenic Synergy MS-ORT

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  • SALMONELLA SUMIT 2013: Technologies for Salmonella Control in Live Production

    We present here the link to the first conference enlarged on Salmonella, organized by Poultry Federation, focusing on The control from production to the consumer. It was held on 26 and 27 March. Here we provide links to the presentations of Dr. Tellez, belonging to Pacific Vet Group and the University of Arkansas.

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  • Salmonella Sumit 2013: Why We Haven't Fixed The Salmonella Problem

    The papers of Salmonella Summit are availables on-line.

    We present here the link to the first conference enlarged on Salmonella, organized by Poultry Federation, focusing on The control from production to the consumer. It was held on 26 and 27 March. Here we provide links to the presentations of Dr. Barton, belonging to Pacific Vet Group and the University of Arkansas.

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